BIOL 2120:
The lab covers basics of culture and identification of bacteria and microbial ecology and covers basics of microbiology.
Corequisites
Must register for both lecture and lab
In the event of an emergency or announced campus closure due to a natural disaster or pandemic, it may be necessary for Panola College to move to altered operations. During this time, Panola College may opt to continue delivery of instruction through methods that include, but are not limited to: online learning management system (CANVAS), online conferencing, email messaging, and/or an alternate schedule. It is the responsibility of the student to monitor Panola College's website (www.panola.edu) for instructions about continuing courses remotely, CANVAS for each class for course-specific communication, and Panola College email for important general information.
Regular and punctual attendance of classes and laboratories is required of all students. When a student has been ill or absent from class for approved extracurricular activities, he or she should be allowed, as far as possible, to make up for the missed work. If a student has not actively participated by the census date, they will be dropped by the instructor for non-attendance. This policy applies to courses that are in-person, online, hybrid, and hyflex.
Attendance in online courses is determined by submission of an assignment or participation in an activity. According to federal guidelines, simply logging into a distance learning course without participating in an academic assignment does not constitute attendance. Distance learning is defined as when a majority (more than 50%) of instruction occurs when the instructor and students are in separate physical locations. Students must engage in an academic activity prior to the course census date.
When an instructor feels that a student has been absent to such a degree as to invalidate the learning experience, the instructor may recommend to the Vice President of Instruction that the student be withdrawn from the course. Instructors may seek to withdraw students for non-attendance after they have accumulated the following number of absences:
Fall or spring semesters:
3 or more class meeting times per week - 5 absences
2 class meeting times per week - 3 absences
1 class meeting per week - 2 absences
The student is responsible for seeing that he or she has been officially withdrawn from a class. A student who stops attendance in a class without officially withdrawing from that class will be given a failing grade; consequently, the student must follow official withdrawal procedures in the Admissions/Records Office.
Please note: Health Science and Cosmetology courses may require more stringent attendance policies based on their accreditation agencies. Please see the addendum and/or program handbook for further information concerning attendance.
Panola College welcomes pregnant and parenting students as a part of the student body. This institution is committed to providing support and adaptations for a successful educational experience for pregnant and parenting students. Students experiencing a need for accommodations related to pregnancy or parenting will find a Pregnancy and Parenting Accommodations Request form in the Student Handbook or may request the form from the course instructor.
No use of Generative AI permitted.
This option assumes that all work submitted by students will be generated by the students themselves, whether they are working individually or in groups. Students should not have another person or entity do the writing of any portion of an assignment, which includes hiring a person or a company to write assignments and/or using artificial intelligence (AI) tools like ChatGPT. Use of any AI-generated content in this course qualifies as academic dishonesty and violates Panola College’s standards of academic integrity.
The purpose of this course is to familiarize the student with the concepts, principles and theories of science and provide an opportunity to experience and appreciate the processes and methodology of science.
Laboratory:
After studying all materials and resources presented in the course, the student will be able to:
- Use and comply with laboratory safety rules, procedures, and universal precautions.
- Demonstrate proficient use of a compound light microscope.
- Describe and prepare widely used stains and wet mounts, and discuss their significance in identification of microorganisms.
- Perform basic microbiology procedures using aseptic techniques for transfer, isolation and observation of commonly encountered, clinically significant bacteria.
- Use different types of bacterial culture media to grow, isolate, and identify microorganisms.
- Perform basic bacterial identification procedures using biochemical tests.
- Estimate the number of microorganisms in a sample using methods such as direct counts, viable plate counts, or spectrophotometric measurements.
- Demonstrate basic identification protocols based on microscopic morphology of some common fungi and parasites.
A general description of lab/discussion topics included in this course are listed in the Learning Outcomes section of this syllabus. Students in all sections of this course will learn the following content:
Upon the successful completion of this course, the student should have an understanding of the following specific laboratory course objectives:
- Identify and demonstrate proper safety procedures concerning laboratory safety.
- Apply scientific reasoning to investigate and analyze collected data.
- Identify the parts and function of the microscope.
- Understand proper use of the microscope, including technique for oil immersion.
- Demonstrate the ability to properly prepare slides for microscopic examination.
- Understand the role of proper aseptic technique in the handling of microorganisms.
- Identify the purpose and principles associated with negative, simple, Gram, capsule, acid-fast, and endospore staining.
- Discuss the importance of constructing wet mounts, streak plates, and spread plates.
- Estimate microbial numbers in a sample using serial dilution techniques.
- Understand the effect of environmental factors on microbial growth as related to cell structure and metabolism (osmotic pressure, temperature, oxygen requirements, and pH).
- Identify the purpose and principles associated with different media types utilized in the laboratory.
- Understand the purpose and principles associated with biochemical test media in determining metabolic differences between microbes.
- Utilize resources such as Bergey’s Manual to correctly identify an unknown bacterial strain.
- Understand the role of chemical and physical control of microbes (antibiotics and disinfectants).
This course is offered in the traditional face-to-face classroom environment and online.
- Students will have access to this course using Canvas, a learning management system. Student learning outcomes, outlines/notes, power points, reviews, videos, and other study aids are provided within Canvas.
- A Connect Microbiology access code (provides access to the publisher’s digital learning environment that helps to improve performance) is required to provide access to an eBook and homework (participation) assignments that help in the understanding of learning outcomes. Connect also contains the SmartBook study tool that can help improve memory recall and increase student performance and retention. The Connect Microbiology access code also provides quizzes and demonstration labs for enhancing certain laboratory topics for both face-to-face and online students.
- Students completing a face-to-face lab will follow class attendance guidelines as indicated in the Panola College catalog with required attendance to laboratory sessions.
- Students that are participating in an online laboratory are responsible for properly completing and submitting assignments by requested due dates, and properly following clean-up instructions associated with their Lab Kit.
- Online students will be completing your major online lab quizzes if you are in the online lab using the Respondus Online Testing program within Canvas. Some requirements for Respondus include a computer with a camera/Webcam, Google Chrome as your browser, reliable Internet service, and a quiet location. Online students may also choose to complete their exams at an On-Campus Testing Center (Carthage, Marshall, or Center). Hours of operation of the On-Campus testing centers are posted on the Student Support Services webpage (Student Support | Panola College). Examinations will require approximately 60 minutes for completion and the completion time for quizzes vary (is much shorter) depending on content.
- Students should feel free to email any questions or concerns associated with this course to the instructor. Please be sure to check and appropriately respond to your emails in Canvas. Students should also feel free to communicate by phone or in person during scheduled office hours.
- Students in both the traditional and online classes should use e-mail within Canvas to communicate with the instructor. Using Canvas email gives you access to the instructor and other classmates--you just select a name from the list. If there is an issue in contacting your instructor using email in Canvas, you may use their Panola College email address (located in the “Getting Started” module in Canvas). I will attempt to respond to all emails within 24 hours.
- Students are expected to demonstrate academic integrity. Evidences of any form of cheating (from lecture or lab assessments to examinations), plagiarism, collusion, or inappropriate use of artificial intelligence (AI) will result in a grade of a 0 for that assignment. Completing online examinations using the Respondus Online testing service requires the student to follow provided instructions/guidelines. Deviating from these instructions/guidelines will result in a grade of a 0 for that exam/quiz evaluation.
o Academic integrity during the testing process is the student’s responsibility and has to be maintained. The testing experience at your location should mimic the testing environment in a face-to-face classroom. Some of the major guidelines include a proper 360-degree room scan, maintaining consistent eye contact with your computer screen, no other individuals should be present in your immediate testing vicinity (unless you are testing in a public place), and there should not be conversations with others during the exam (unless you are completing your exam with a reader (through Student Disability Services). Using the Respondus program is an advantage to provide a degree of flexibility and convenience to your schedule. Misuse of this privilege will result in a grade of a 0 for an exam/quiz evaluation and will require that you complete your future exams/quizzes in a face-to-face proctored environment (testing center).
o Further information concerning proper online testing instructions/guidelines are provided within your Respondus module within Canvas. Technical issues related to internet service or computer glitches/problems occurring during proctored online evaluations (quizzes/examinations) may require the student to complete these evaluations face-to face at an On-Campus Testing Center.
- A course information sheet will be provided to all students and will include instructor information, course requirements, information on academic integrity, testing information, grading information, course materials, strategies for success, and a tentative schedule. The course information sheet will be located in Canvas.
Laboratory:
- Laboratory quizzes and laboratory exercises will provide an understanding of a number of microbiology concepts and activities (including microscope use, stain procedures, streak and spread plates, factors influencing microbial growth, culture transfers, antibiotic sensitivity, and bacterial/protozoan structure).
- Determination of a microbiology unknown using a variety of morphological and biochemical tests. This activity will involve data collection, using a dichotomous key, and critical thinking.
Laboratory
The laboratory component of the microbiology course is 30% of your total grade.
- Laboratory activities may include quizzes, drawings, laboratory reports, or any other methodologies deemed important by the instructor. Face-to-face lab students must be both present and participating in the lab activity. Lab assessments are generally due the next scheduled lab class after a laboratory activity has been completed but due dates will be provided within your provided lab agenda. Late work is not accepted. If the student misses a laboratory activity/assignment, the instructor should be contacted ASAP to develop an alternate schedule. For lab activities and quizzes, legitimate missed labs should be scheduled and completed within a week of the missed lab class. The quantity of materials necessary and the time sensitive nature of some exercises are usually not conducive for make-up sessions. Online lab students will need to evaluate their instructions for a specific lab activity and use proper time management skills to complete their lab activity and properly submit their work prior to the due date. Ample time will be provided for the completion of lab assignments in the online lab so proper time management skills will be essential. Due dates are provided in posted lab agendas/emails in both the face-to-face and online labs for lab quizzes and lab assessments. Late work is not accepted.
- The microbiology unknown assignment is a major assessment in the microbiology lab and involves determining the identity of an unknown microorganism. The assessment grade sheet evaluates streak plate technique, proper aseptic technique, subculturing, Gram staining, interpreting a number of biochemical tests, data collection, and constructing a dichotomous key. The dichotomous key allows the selection of the potential unknown organism which the student will submit to the instructor.
The laboratory component of the microbiology course is 30% of your total course grade. Within the laboratory component of the course:
- Laboratory quizzes and exercises represent ~79% of your lab grade. Failure to follow Respondus guidelines/instructions can result in a grade of a 0 for an online quiz/examination and will require that you complete your future quizzes/exams in a face-to-face proctored environment (testing center).
- The microbiology unknown represents ~20% of your lab grade. Cheating of any form will result in a grade of a 0 on any lab assessment.
Grade Determination:
Final course grades are determined by the following scale:
A=100-90 B=89-80 C=79-70 D=69-60 F=< 59.5
Online laboratory students (only) will need the Connect Online Access Code (above).
- Courses conducted via video conferencing may be recorded and shared for instructional purposes by the instructor.
- For current texts and materials, use the following link to access bookstore listings: https://www.panolacollegestore.com.
- For testing services, use the following link: https://www.panola.edu/student-services/studentsupport/academic-testing-center.
- The Accommodations & Disability Support (A&DS) Office at Panola College provides and facilitates support services and accommodations for students with disabilities. The A&DS office works under the federal guidelines included in Section 504 of the Rehabilitation Act of 1973 and the American with Disabilities Act. Please contact the Accommodations & Disability Support (A&DS) Office located in the Charles C. Matthews Student Center or go to https://www.panola.edu/disabilitysupport for more information.
- Withdrawing from a course is the student’s responsibility. Students who do not attend class and who do not withdraw will receive the grade earned for the course.
- Student Handbook (located in the Panola College Catalog)